A STUDY ON OXIDIZING EFFECT OF AN EYE LUBRICANT MIXTURE OF CARBOXY METHYL CELLULOSE AND HYALURONATE ON THE TEAR FILM IN HUMAN EYE USING CYCLIC VOLTAMMETRY

The study of the effects of the eye drops used in the two types of a sterile compounds (carboxy methyl cellulose sodium 0.5% (CMCS 0.5%) and sodium hyaluronate 0.2% (SH0.2%)) using electrochemical method by cyclic voltammetry in the natural media at pH=6.8 which is the same as tear film pH. This study aimed to evaluate the activity of both eye drops (carboxy methyl cellulose sodium 0.5% (CMCS0.5%) and sodium hyaluronate 0.2% (SH0.2%)) and redox effects on the eyes in tear film resembling media and blood medium.

The results were given for the CMCS0.5% in normal saline as an electrolyte in the cell of cyclic voltammetric technique, two cathodic current peaks of CMCS0.5% at 250 mV and -250 mV, While the result of voltammogram in distill water single reduction current peak of CMCS0.5% at -600 mV. But in blood medium the CMCS0.5% shows reduction current peak at -900 mV. The same antioxidants current peak appeared in KCl as an electrolyte at -100 mV, so the CMCS0.5% acts as antioxidant in the eye lubricant on the tear film in human eye and in different electrolytes.

The other compound of sterile eye drop is SH0.2% which was studied in normal saline that showed two oxidation current peaks at 250 mV and 500 mV and two reduction current peaks at 400 mV and -500 mV. While in the blood medium, it made one oxidation current peak at 1mV and other reduction current peak at -750 mV.

The both CMCS0.5% and SH0.2% eye drops can be used properly as a safe sterile eye drop regarding the chemical composition as they act as antioxidant agents in the neutral medium as in the tear media of human eyes.