Anti-urease Activity of Mimusops elengi Linn (Sapotaceae)

Aim: Mimusops elengi L. (Sapotaceae) commonly known as Bakul is a small to large evergreen tree found all over the different parts of the world. Literature survey showed no reports on the anti urease activity of the flowers and leaves of Mimusops elengi. The present study was therefore envisaged to evaluate the anti urease activity of the methanolic extracts (MFE and MLE) of M. elengi.

Study Design: Assessment of anti urease activity and total phenolic contents.

Methodology: For the determination of total phenolic content of M. elengi extracts (MFE and MLE) the reaction mixture contained 50% Folin-Ciocalteu reagent (0.5 mL), 20% (w/v) sodium carbonate solution (2.5 mL), and gallic acid solution (1, 2.5, 5, 10, 25, 50, 100 mg/ml) or sample extracts (1.0 mL). Urease inhibition activity was determined by mixing Urease (Jack bean) method was employed for urease inhibition activity.

Results: From the standard calibration curve of gallic acid the total phenolic contents in extracts (MFE and MLE) was found to be 7.5±0.28 mg/g, 96.8±4.6 mg/g. Urease inhibitory activity of M. elengi extracts (MFE and MLE) was comparable with standard Thiourea. The percentage urease inhibition of Thiourea was found to be 98.2%, IC50 88.2 ± 0.01 mg/ml. The maximum urease inhibitory activity was shown by the leaves extract (MLE) i. e, 77.9% (IC50 62.1±1.20 mg/ml) while methanolic extract of flower (MFE) produced 47.6% urease inhibition activity. The total phenolic contents in both methanolic extracts (MFE and MLE) was found to be 7.5±0.28 mg/g, 96.8±4.6 mg/g respectively.

Conclusion: It is, therefore, concluded that this plant can be a sources to isolate some natural urease inhibitory agents.