Gene Expression Related to Physiological Quality of Soybean Seeds

Moreno, Kiliany A. A. and Pires, Raquel M. O. and Castro, Maria L. R. and Vasconcellos, Renato C. C. and Santos, Heloisa O. and Pinho, Édila V. R. V. (2019) Gene Expression Related to Physiological Quality of Soybean Seeds. Journal of Agricultural Science, 11 (3). p. 370. ISSN 1916-9752

[thumbnail of 5c4831bc1a4e9.pdf] Text
5c4831bc1a4e9.pdf - Published Version

Download (1MB)

Abstract

Production of soybeans with high physiological quality is important to ensure the productivity of improved plant populations. The objective of this work was to select soybean genotypes for high physiological quality of seeds, through germination and vigor tests, and to study gene expression by transcripts and proteins. In a first trial, seeds of 12 soybean cultivars were evaluated and selected six cultivars with contrasting physiological quality levels. At the protein level, the isoenzyme systems alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), sorbitol dehydrogenase (SDH), superoxide dismutase (SOD), catalase (CAT), isocitrate lyase (ICL), esterase (EST), glutamate oxaloacetate transferase (GOT), and heat-resistant proteins were evaluated. For the transcript levels, the real-time PCR technique (qRT-PCR) was used, and the genes coding for the SOD, CAT, MDH, PGI, ICL and PRX enzymes were studied. The germination and vigor tests classified the seeds of cultivars CD201, CA115 and MS8400 as high quality, while the cultivars Syn1263, Syn1279 and CD202 were classified as of low quality. The enzymes involved in the process such as dehydrogenase and phosphoglucose isomerase, are promising markers for assessing the physiological quality of soybean seeds. Higher expression of peroxiredoxin enzyme is related to the low physiological quality of soybean seeds.

Item Type: Article
Subjects: European Scholar > Agricultural and Food Science
Depositing User: Managing Editor
Date Deposited: 10 May 2023 10:42
Last Modified: 30 Jan 2024 06:33
URI: http://article.publish4promo.com/id/eprint/1679

Actions (login required)

View Item
View Item