A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration

Humenik, Filip and Danko, Ján and Krešáková, Lenka and Vdoviaková, Katarína and Vrabec, Vladimír and Vasilová, Emília and Giretová, Mária and Tóth, Štefan and Fagová, Zuzana and Babík, Ján and Medvecký, Ľubomír (2023) A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration. International Journal of Molecular Sciences, 24 (22). p. 16080. ISSN 1422-0067

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Abstract

The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting.

Item Type: Article
Subjects: European Scholar > Medical Science
Depositing User: Managing Editor
Date Deposited: 09 Nov 2023 06:34
Last Modified: 09 Nov 2023 06:34
URI: http://article.publish4promo.com/id/eprint/2835

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